goat anti glutamate decarboxylase 67 gad67  (R&D Systems)

Journal: Frontiers in Neural Circuits

Article Title: Dorsal striatum c-Fos activity in perseverative ephrin-A2A5 −/− mice and the cellular effect of low-intensity rTMS

doi: 10.3389/fncir.2023.1179096

Figure Lengend Snippet: c-Fos activation in DMS and DLS across rTMS treated and untreated wildtype and ephrin-A2A5 −/− mice. Total c-Fos density (cells/mm 3 ) (A, B) ; % of c-Fos + cells colocalized with DARPP32 + cells (C, D) ; unspecified c-Fos + cells (E, F) ; and c-Fos + cells colocalized with GAD67 + -DARPP32 − (G, H) . Points represent individual animal counts. Overall, c-Fos activation did not differ between strains or treatment groups. However, the significant omnibus test indicated a difference in DMS c-Fos-DARPP32 colocalization. Results suggested that in ephrin-A2A5 −/− , rTMS increased the percentage of c-Fos-DARPP32 colocalization but pairwise post hoc comparisons did not survive multiple corrections.

Article Snippet: These sections were stained for three different antibodies: c-Fos (rabbit polyclonal c-Fos antibody, 1:5000, Abcam, ab190289), a marker for neuronal activity (Bullitt, ); cAMP-regulated phosphoprotein-32 kD (DARPP32) (purified mouse anti-DARPP32, 1:2000, BD Transduction Laboratories, 611520), a marker for MSNs (Anderson and Reiner, ); and glutamate decarboxylase 67 (GAD67) (anti-Goat, 1:750, R&D systems, AF2086-SP), a marker for GABAergic neurons (Lazarus et al., ).

Techniques: Activation Assay

Journal: bioRxiv

Article Title: Isolated loss of the AUTS2 long isoform, brain-wide or targeted to Calbindin -lineage cells, generates a specific suite of brain, behavioral and molecular pathologies

doi: 10.1101/2023.05.04.539486

Figure Lengend Snippet: Hippocampal PV+ interneurons express AUTS2 and are reduced in numbers in in Nes-cre, ex7 -/- mice , abbreviated as ex7 -/- in figures. a) Hippocampal PV+ cells were significantly decreased in all HC regions in Nes-cre, ex7 -/- mutants compared to WT littermates, as were overall counts. b) In the cortex and HC of adult wildtype mouse, PV+ cells (green) showed abundant AUTS2 co-expression (red) . Red boxes show zoomed in view of indicated area. c) In P14 cortex and HC, some (white arrows and red zoom boxes) but not all (white box) GAD+ interneurons (green) co-expressed AUTS2 expression (red) at high levels. Cursor and white arrows show examples of overlap of GAD+ AUTS2+ cells. Red boxes show zoomed in view of indicated area. d) In adult DG, GAD+ cells (green) showed abundant expression of AUTS2 (red). Red boxes show zoomed in view of indicated area. e) The ratio of cFOS+/total cell count in CA1 was significantly increased in adult mutants compared to WT. Two-tailed t-test was used to test statistical significance, * = p<0.05, ** = p<0.01, ***= p<0.001, **** = p<0.0001.

Article Snippet: Primary antibodies: AUTS2 (Sigma, HPA000390), Calbindin D- 28K (Sigma C9848, 1:300), Calbindin (Santa Cruz sc-365360, 1:300), Calretinin (Santa Cruz sc-365989, 1:300), Calretinin (ZYM MD, 1:300), Tbr2 (Invitrogen 14-4875-52, 1:100), Parvalbumin (R&D system AF5058, 1:50 – 1:100), cFOS (Santa Cuz s-166940, 1:200), GAD (R&D system AF2086-SP, 1:100); Secondary antibodies (Thermo-Fisher Scientific): Goat anti-mouse IgG (Alexa Fluor 488, A11001), Goat anti-Rabbit IgG (Alexa Fluor 594, A11012), Donkey anti-Rat IgG (Alexa Fluor 594, A21209), Donkey anti-Sheep IgG (Alexa Fluor 594, abcam, ab150180), Donkey anti-Sheep IgG (Novus Biologicals, DyLight488, NBP173002).

Techniques: Expressing, Cell Counting, Two Tailed Test

Journal: bioRxiv

Article Title: Isolated loss of the AUTS2 long isoform, brain-wide or targeted to Calbindin -lineage cells, generates a specific suite of brain, behavioral and molecular pathologies

doi: 10.1101/2023.05.04.539486

Figure Lengend Snippet: Hippocampal PV+ interneurons express AUTS2 and are reduced in numbers in in Nes-cre, ex7 -/- mice , abbreviated as ex7 -/- in figures. a) Hippocampal PV+ cells were significantly decreased in all HC regions in Nes-cre, ex7 -/- mutants compared to WT littermates, as were overall counts. b) In the cortex and HC of adult wildtype mouse, PV+ cells (green) showed abundant AUTS2 co-expression (red) . Red boxes show zoomed in view of indicated area. c) In P14 cortex and HC, some (white arrows and red zoom boxes) but not all (white box) GAD+ interneurons (green) co-expressed AUTS2 expression (red) at high levels. Cursor and white arrows show examples of overlap of GAD+ AUTS2+ cells. Red boxes show zoomed in view of indicated area. d) In adult DG, GAD+ cells (green) showed abundant expression of AUTS2 (red). Red boxes show zoomed in view of indicated area. e) The ratio of cFOS+/total cell count in CA1 was significantly increased in adult mutants compared to WT. Two-tailed t-test was used to test statistical significance, * = p<0.05, ** = p<0.01, ***= p<0.001, **** = p<0.0001.

Article Snippet: Primary antibodies: AUTS2 (Sigma, HPA000390), Calbindin D- 28K (Sigma C9848, 1:300), Calbindin (Santa Cruz sc-365360, 1:300), Calretinin (Santa Cruz sc-365989, 1:300), Calretinin (ZYM MD, 1:300), Tbr2 (Invitrogen 14-4875-52, 1:100), Parvalbumin (R&D system AF5058, 1:50 – 1:100), cFOS (Santa Cuz s-166940, 1:200), GAD (R&D system AF2086-SP, 1:100); Secondary antibodies (Thermo-Fisher Scientific): Goat anti-mouse IgG (Alexa Fluor 488, A11001), Goat anti-Rabbit IgG (Alexa Fluor 594, A11012), Donkey anti-Rat IgG (Alexa Fluor 594, A21209), Donkey anti-Sheep IgG (Alexa Fluor 594, abcam, ab150180), Donkey anti-Sheep IgG (Novus Biologicals, DyLight488, NBP173002).

Techniques: Expressing, Cell Counting, Two Tailed Test